human trit1 Search Results


promoter reporter clone for human trit1  (Genecopoeia)
N/A
Using a secreted and robust Gaussia Luciferase (GLuc) as the reporter, GeneCopoeia GLuc-ON™ promoter clones are designed for promoter analysis by detecting the real-time activities of about 39,500 human, 28,700 mouse and 17,500 rat promoters
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hek293  (OriGene)
93
OriGene hek293
(a) Relative normalized expression of Trit1 analysis using real-time qPCR for Trit1 KO validation, n =3, unpaired t -test * p < 0.05. (b) Venn diagram showing the decreased number of i 6 A sites found in mESCs Trit1 KO compared to control. (c) and (d) Analysis of TRIT1 OE after transfection with TRIT1 plasmid in <t>HEK293</t> cells, using real-time qPCR (c), n =3, unpaired t -test, * p <0.05 and WB (d). (e) Venn diagram showing the overlap of i 6 A sites found in HEK293 TRIT1 OE and control in mRNA samples. (f-g) LC-MS/MS quantification of i 6 A/A ratio in HEK293 control and TRIT1 OE in small RNA (f) and mRNA (g), n =3, unpaired t -test *p < 0.05. (h) i 6 A sites in TRIT1 OE compared to control cells, represented according to their segment location in the transcript. (i) Sequence logo representing the deduced consensus of i 6 A sites in TRIT1 OE cells.
Hek293, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+trit1/TRIT1+(NM_017646)+Human+Tagged+ORF+Clone/bio_rxiv__2025__11__26__690722-33-13-40
Average 93 stars, based on 1 article reviews
hek293 - by Bioz Stars, 2026-09
93/100 stars
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lentiviral plasmids  (OriGene)
90
OriGene lentiviral plasmids
(a) Relative normalized expression of Trit1 analysis using real-time qPCR for Trit1 KO validation, n =3, unpaired t -test * p < 0.05. (b) Venn diagram showing the decreased number of i 6 A sites found in mESCs Trit1 KO compared to control. (c) and (d) Analysis of TRIT1 OE after transfection with TRIT1 plasmid in <t>HEK293</t> cells, using real-time qPCR (c), n =3, unpaired t -test, * p <0.05 and WB (d). (e) Venn diagram showing the overlap of i 6 A sites found in HEK293 TRIT1 OE and control in mRNA samples. (f-g) LC-MS/MS quantification of i 6 A/A ratio in HEK293 control and TRIT1 OE in small RNA (f) and mRNA (g), n =3, unpaired t -test *p < 0.05. (h) i 6 A sites in TRIT1 OE compared to control cells, represented according to their segment location in the transcript. (i) Sequence logo representing the deduced consensus of i 6 A sites in TRIT1 OE cells.
Lentiviral Plasmids, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+trit1/TRIT1+Human+shRNA+Plasmid+Kit/pmc08070726-58-0-7
Average 90 stars, based on 1 article reviews
lentiviral plasmids - by Bioz Stars, 2026-09
90/100 stars
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trit1 human qpcr template standard  (OriGene)
N/A
qPCR primer pairs and template standards against Homo sapiens gene TRIT1
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human trit1 activation kit by crispra  (OriGene)
N/A
TRIT1 CRISPRa kit CRISPR gene activation of human tRNA isopentenyltransferase 1
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trit1 nm 017646 human recombinant protein  (OriGene)
N/A
Recombinant protein of human tRNA isopentenyltransferase 1 TRIT1
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trit1 nm 017646 human tagged orf clone  (OriGene)
N/A
Lenti ORF clone of Human tRNA isopentenyltransferase 1 TRIT1 Myc DDK tagged
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trit1 (nm_017646) human over-expression lysate  (Boster Bio)
N/A
Transient overexpression lysate of tRNA isopentenyltransferase 1 (TRIT1)
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trit1 nm 017646 human untagged clone  (OriGene)
N/A
TRIT1 untagged Human tRNA isopentenyltransferase 1 TRIT1
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trit1 human qpcr primer pair  (OriGene)
N/A
qSTAR qPCR primer pairs against Homo sapiens gene TRIT1
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rabbit anti-human trit1 polyclonal antibody  (Cusabio)
N/A
Rabbit anti-Human TRIT1 Polyclonal Antibody
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Image Search Results


(a) Relative normalized expression of Trit1 analysis using real-time qPCR for Trit1 KO validation, n =3, unpaired t -test * p < 0.05. (b) Venn diagram showing the decreased number of i 6 A sites found in mESCs Trit1 KO compared to control. (c) and (d) Analysis of TRIT1 OE after transfection with TRIT1 plasmid in HEK293 cells, using real-time qPCR (c), n =3, unpaired t -test, * p <0.05 and WB (d). (e) Venn diagram showing the overlap of i 6 A sites found in HEK293 TRIT1 OE and control in mRNA samples. (f-g) LC-MS/MS quantification of i 6 A/A ratio in HEK293 control and TRIT1 OE in small RNA (f) and mRNA (g), n =3, unpaired t -test *p < 0.05. (h) i 6 A sites in TRIT1 OE compared to control cells, represented according to their segment location in the transcript. (i) Sequence logo representing the deduced consensus of i 6 A sites in TRIT1 OE cells.

Journal: bioRxiv

Article Title: i 6 A-seq maps N 6 -isopentenyladenosine and uncovers its role as a regulator of mRNA stability through recruitment of DIS3L2

doi: 10.1101/2025.11.26.690722

Figure Lengend Snippet: (a) Relative normalized expression of Trit1 analysis using real-time qPCR for Trit1 KO validation, n =3, unpaired t -test * p < 0.05. (b) Venn diagram showing the decreased number of i 6 A sites found in mESCs Trit1 KO compared to control. (c) and (d) Analysis of TRIT1 OE after transfection with TRIT1 plasmid in HEK293 cells, using real-time qPCR (c), n =3, unpaired t -test, * p <0.05 and WB (d). (e) Venn diagram showing the overlap of i 6 A sites found in HEK293 TRIT1 OE and control in mRNA samples. (f-g) LC-MS/MS quantification of i 6 A/A ratio in HEK293 control and TRIT1 OE in small RNA (f) and mRNA (g), n =3, unpaired t -test *p < 0.05. (h) i 6 A sites in TRIT1 OE compared to control cells, represented according to their segment location in the transcript. (i) Sequence logo representing the deduced consensus of i 6 A sites in TRIT1 OE cells.

Article Snippet: Plasmids coding WT or mutant FLAG-tagged human TRIT1 (Origene, RC210476) were transfected into HEK293 and DMS273-shTRIT1 cells using TransIT-X2 reagent (Mirus Bio), according to manufacturer instructions, and harvested after 48 hours. siRNAs including scramble control or siRNA directed against DIS3L2 (Origene, SR315069) were transfected twice into DMS273-Scramble cells using RNAiMAX reagent (Invitrogen), according to manufacturer instructions, every 48 hours.

Techniques: Expressing, Biomarker Discovery, Control, Transfection, Plasmid Preparation, Liquid Chromatography with Mass Spectroscopy, Sequencing

(a) Real-time qPCR showing TRIT1 OE effect on gene expression in highly isopentenylated genes in HEK293 cells, n =3, unpaired t -test, * p <0.05. (b) Real-time qPCR of the indicated genes in shTRIT1 cells compared to their control, before and after i 6 A-IP, n =3. (c-d) WB of the indicated cell lines, after TRIT1 OE/KD, displaying an inverse change in SLC38A10 (c) and AIG1 (d) in response to shifts in TRIT1 protein expression. (e) Immunofluorescence staining images of SLC38A10 and AIG1 in scramble and shTRIT1 DMS273 cells, revealing their increased expression under TRIT1 KD. (f) Analysis of TRIT1, SLC38A10 and AIG1 after TRIT1 WT or mutant rescue in shTRIT1 cells by real-time qPCR, n =3, unpaired t -test, * p <0.05. (g) WB of the indicated cell lines, representing similar trend for TRIT1 and SLC38A10 as in (f). (h) Real-time qPCR of SLC38A10 and AIG1 mRNA levels, in DMS273 scramble versus shTRIT1, monitored 6 hours after Actinomycin-D addition, compared to 0 hours, n =3.

Journal: bioRxiv

Article Title: i 6 A-seq maps N 6 -isopentenyladenosine and uncovers its role as a regulator of mRNA stability through recruitment of DIS3L2

doi: 10.1101/2025.11.26.690722

Figure Lengend Snippet: (a) Real-time qPCR showing TRIT1 OE effect on gene expression in highly isopentenylated genes in HEK293 cells, n =3, unpaired t -test, * p <0.05. (b) Real-time qPCR of the indicated genes in shTRIT1 cells compared to their control, before and after i 6 A-IP, n =3. (c-d) WB of the indicated cell lines, after TRIT1 OE/KD, displaying an inverse change in SLC38A10 (c) and AIG1 (d) in response to shifts in TRIT1 protein expression. (e) Immunofluorescence staining images of SLC38A10 and AIG1 in scramble and shTRIT1 DMS273 cells, revealing their increased expression under TRIT1 KD. (f) Analysis of TRIT1, SLC38A10 and AIG1 after TRIT1 WT or mutant rescue in shTRIT1 cells by real-time qPCR, n =3, unpaired t -test, * p <0.05. (g) WB of the indicated cell lines, representing similar trend for TRIT1 and SLC38A10 as in (f). (h) Real-time qPCR of SLC38A10 and AIG1 mRNA levels, in DMS273 scramble versus shTRIT1, monitored 6 hours after Actinomycin-D addition, compared to 0 hours, n =3.

Article Snippet: Plasmids coding WT or mutant FLAG-tagged human TRIT1 (Origene, RC210476) were transfected into HEK293 and DMS273-shTRIT1 cells using TransIT-X2 reagent (Mirus Bio), according to manufacturer instructions, and harvested after 48 hours. siRNAs including scramble control or siRNA directed against DIS3L2 (Origene, SR315069) were transfected twice into DMS273-Scramble cells using RNAiMAX reagent (Invitrogen), according to manufacturer instructions, every 48 hours.

Techniques: Gene Expression, Control, Expressing, Immunofluorescence, Staining, Mutagenesis